Example data

Basic information:

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• Full WT sequence: AAACGCCCATGCAATTAGTCTATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGTGATGGCAGAGGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACTGGTTGACAAGTTTGGCTGG
• Target DNA sequence (SpCas9, without PAM): GGCCCAGACTGAGCACGTGA
• Mutation pattern: Ins
• Mutation position: 1
• Mutation sequence: CTT
• Additional nicking target sequence for PE3 (5' to 3', without PAM): GGACAGCTTTTCCTAGACAG

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