CRISPR RGEN Tools

CReVIS-Seq

A CRISPR enhanced Viral Integration Site detection method in genome level

We describe CReVIS-seq, a highly efficient, genome-wide method for detecting viral insertion sites using high-throughput sequencing, which is based on in vitro circularization and subsequent cleavage of genomic DNAs in a CRISPR guide RNA-specific manner.

Citation info: Kim HS. et al. CReVIS-seq: a highly accurate and multiplexable method for genome-wide mapping of lentiviral integration sites

Please input your data in below form, or download an example.

Download an off-line version of CReVIS-Seq here.

Sequencing Data

Read 1 (fastq or gzipped fastq):

Read 2 (fastq or gzipped fastq):

Basic Information
Lentiviral long terminal repeats (LTR) sequence (5' to 3'):

sgRNA sequence for in vitro cleavage (5' to 3', without PAM)

Junction position

Analysis Parameters
Alignment range (R) (30 < R < 70)[?]

Minimum frequency (n) [?]

Input Summary

LTR sequence
(blue: indicator sequences at each ends of comparision range, green: LTR sequence in data)

crRNA sequence

Comparison range (R) [?] Minimum frequency (n) [?]

Progress

Edited data

Initializing...

Input Summary

WT sequence
(blue: indicator sequences at each ends of comparision range, green: crRNA sequence, red: WT marker sequence)

crRNA sequence

Comparison range (R) [?] Minimum frequency (n) [?]

Result Summary

LTR sequence (blue: indicator sequences at each ends of comparision range, green: LTR sequence in data)


crRNA sequence

Comparison range (R) [?]			Minimum frequency (n) [?]