CRISPR RGEN Tools

PE-Analyzer

A JavaScript-based instant assessment tool for high-throughput sequencing data for CRISPR prime edited cells.

Prime editing tools that consist of a reverse transcriptase linked with Cas9 nickase are capable of generating targeted insertions, deletions, and base conversions without producing DNA double strand breaks or requiring any donor DNA. Thanks to the improvements in the newest JavaScript engines in the most recent web browsers, the JavaScript based internal algorithm of PE-Analyzer completely runs on the client-side so that large amounts of sequencing data do not need to be uploaded to the server.

"Citation info: ""Hwang G.H. "et al". PE-Designer and PE-Analyzer: web-based design and analysis tools for CRISPR prime editing "Nucleic Acid Resaerch", gkab319 (2021)."

Please input your data in below form, or download an example data here.

Sequencing Data slot count

File Type:

You can see your sequence information when input the basic information.

Basic Information

Full reference sequence (5' to 3'):

Select Nuclease:

pegRNA spacer sequence (5' to 3', without PAM sequence):

Mutation pattern

Position (>= 1)

Mutation sequence

(Optional) Additional nicking target sequence for PE3 or PE3b system (5' to 3', without PAM sequence):

Analysis Parameters

Comparison range (R) [?]

or use both ends

Minimum frequency (n) [?]

Prime-edit marker (r) [?]

WT marker for additional nicking target sequence [?]

WT sequence


crRNA sequence

Comparison range (R) [?]	Minimum frequency (n) [?]	Prime-edit marker (r) [?]