CRISPR RGEN Tools

Cas-OFFinder

A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases.

Citation info: Bae S., Park J., & Kim J.-S. Cas-OFFinder: A fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 30, 1473-1475 (2014).

Submit a new searching job, or download an off-line version of Cas-OFFinder here.

Job title (Optional):

E-mail (Optional):

* The result will be notified by e-mail (searching job is working in sequence for many input data, therefore it would be convenient to receive the results by e-mail).

Query Sequences

Query sequences (5' to 3'), one sequence per line.
Please write crRNA sequences without PAM sequences (e.g. without NGG for SpCas9).
The length of each query sequence should be between 15 and 25 nt, and all be the same length!

Please note that large number of bulge size will significantly increase the calculation time!

Mixed bases are allowed.

The count of query sequence must be less than 1000.

(In case of NNN and NRN PAM, must be less than 10.)

Mismatch Number(eq or less than)

DNA Bulge Size(eq or less than)

RNA Bulge Size(eq or less than)